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KMID : 0545120080180010118
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 1 p.118 ~ p.123
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A New Method for Antimicrobial Susceptibility Testing of In Vitro-cultured Bacteria By Means of Resonance Light Scattering Technique
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Shi Yu Jun
Chen Jun
Xu Ming
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Abstract
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A new method for antimicrobial susceptibility testing of in vitro-cultured bacteria on an ordinary fluorescence spectrometer was developed. The viable bacteria reduced 3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) to produce insoluble particles that displayed intense resonance scattering light (RSL). The assay showed a linear relationship between the number of viable bacteria and the intensity of resonance scattering light. Dead bacteria were unable to reduce MTT. Methicillin-resistant Staphylococcus aureus exposed to flavonoids from Marchantia convoluta showed a flavonoids concentration-dependent inhibition of the ability to reduce MTT. In the assay, less than 12 h were required to attain susceptibility results and fewer bacteria were utilized than in traditional methods. The RLS technique could, in combination with the MTT assay, be a rapid and sensitive measuring method to determine the in vitro activity of new antimicrobials.
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KEYWORD
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MTT, antimicrobial susceptibility testing, resonance light scattering
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